Table 1.
antibody | raised in | antigen | type | expected reactivity/specificity |
---|---|---|---|---|
Ab16831 (Abcam) | rabbit | total human WT SOD1 | pAba | total SOD1 |
2770 (Cell Signaling Technology) | rabbit | human SOD1 N-terminal epitope | pAb | total SOD1 |
B8H10 (MédiMabs) | mouse | human G93A SOD1 | mAba | misfolded SOD1 |
C4F6 (MédiMabs) | mouse | human G93A SOD1 | mAb | misfolded SOD1 |
AMF7–63 | rabbit | the electrostatic loop D125DLGKGGNEESTKTGNAG142 | mAb | high affinity for misfolded SOD1 |
3H1 | mouse | the electrostatic loop D125DLGKGGNEESTKTGNAG142 | mAb | 1000-fold lower affinity than AMF7–63 |
10C12 | mouse | SOD1-exposed dimer interface (SEDI)13 with Cys146 replaced by cysteic acid | mAb | oxidized misfolded SOD1 monomers/oligomers/aggregates |
mAb = monoclonal antibody. pAb = polyclonal antibody.