Table 2.

Reactivity of Different Antibodies with WT and G93A

antibody	metalated form reactivitya	reactivity at t = 0 hb	reactivity at t = 4 hb	maximal reactivity time (Figure 4/Figure 5)	maximal reactivityc	reactivity at t = 40 hb,d
WT-SOD1
Ab16831	R	1086 ± 990	1651 ± 1595	8/12	2373 ± 1359	1288 ± 1228
2770	R	1596 ± 727	1980 ± 848	4/4e	1980 ± 848	1336 ± 1195
B8H10	R	1384 ± 367	1973 ± 535	12/12e	2032 ± 699	868 ± 843
C4F6	U	0 ± 0	0 ± 0		0 ± 0
AMF7–63	R	993 ± 293	1672 ± 576	12/40	2316 ± 732	1985 ± 1408
3H1	U	547 ± 108	1188 ± 226	12/24	1834 ± 719	1286 ± 1022
10C12	U	1603 ± 438	4171 ± 1180	8/12	4929 ± 947	2502 ± 1804
G93A-SOD1
Ab16831	R	2238 ± 909	3313 ± 817	12/16	4785 ± 1310	550 ± 171
2770	R	3472 ± 1189	4381 ± 1295	4/16	5182 ± 708	431 ± 347
B8H10	R	2570 ± 845	3166 ± 476	12/16	5285 ± 1636	797 ± 301
C4F6	U	1933 ± 333	1933 ± 166	4/8e	2029 ± 319	1174 ± 664
AMF7–63	R	1779 ± 533	2740 ± 819	12/16	5164 ± 656	1664 ± 997
3H1	U	585 ± 177	1027 ± 259	12/20	3418 ± 1459	1829 ± 1920
10C12	U	3220 ± 803	4099 ± 701	4/8	4919 ± 393	478 ± 248

^aThe metalated form was analyzed separately from the apo form, and therefore, the two cannot be compared quantitatively. Therefore, we only indicate if the metalated form was reactive (R) or unreactive (U).

^bValues are average of the densitometry values shown in Figures 4C,D and 5C,D.

^cThe reactivity at the maximal time each in Figures 4 and 5 was averaged.

^dThe reactivity in fast reactions (Figure 4) was substantially lower than that of slow reactions (Figure 5).

^eThe antibody showed relatively little variation with time.