Table 1. Description of the candidate references, primer sequences and RT-PCR amplification efficiencies in cucumber, pumpkin, and grafted cucumber/pumpkin.
| Gene | Accession number (NCBI) | Annotation | Gene ID in cucumber | Forward primer (5′–3′) | Reverser primer (5′–3′) | Amplification length | Tm(°C) | RT-qPCR efficiency | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Cucumber | Pumpkin | Cucumber/ pumpkin | ||||||||
| ACT | AB010922 | Actin (ACT) | Csa6G484600 | TCTCCGTTTGGACCTTGC | ATTTCCCGTTCGGCAGT | 99 | 83.2 | 0.88 | 1.05 | 0.86 |
| CYP | AY942800 | Cyclophilin | Csa7G009740 | TTTCATGTGCCAGGGAGG | AGCCAATCGGTCTTAGCG | 189 | 88.1 | 0.99 | 1.05 | 1.05 |
| CACS | GW881874 | Clathrin adaptor complex subunit (CACS) | Csa3G902930 | TGGGAAGATTCTTATGAAGTGC | CTCGTCAAATTTACACATTGGT | 171 | 84.2 | 1.02 | 0.95 | 1.00 |
| TUA | AJ715498 | Alpha-tubulin (TUA) | Csa4G000580 | TCAGCGGCAAGGAAGATG | GCGGATTCTGTCCAAGCA | 92 | 83.7 | 1.03 | 0.87 | 1.00 |
| TIP41 | GW881871 | TIP41-like family protein | Csa7G071610 | TGGGAGGATTGCGAGGAGA | AAGTGATATGCCATTGTCAGC | 117 | 81.6 | 0.97 | 1.08 | 1.13 |
| F-BOX | GW881870 | F-box/kelch-repeat protein | Csa5G642160 | TGGTTCATCTGGTGGTCTTG | TTAGCTGCCTCTGCTGATTG | 131 | 84.3 | 1.08 | 0.93 | 0.90 |
| PRL36Aa | HM594174 | 60S ribosomal protein L36a/L44 | Csa3G653380 | AAGATAGTCTTGCTGCACAGGG | AACACGGGCTTGGTTTGA | 79 | 83.3 | 0.97 | 0.95 | 0.99 |
| PP2A | HM594171 | protein phosphatase 2A regulatory subunit A | Csa5G608520 | GAAGCTGTAGGACCTGAACCA | AGCCGCTGCAATACGAAC | 96 | 84.6 | 1.07 | 1.13 | 0.91 |
| UBC | – | – | Csa3G358610 | GTCACCATTCATTTTCCTCCG | GGGCTCCACTGCTCTTTCA | 131 | 83.9 | 1.04 | 1.07 | 1.12 |
| ARF | – | – | Csa5G524710 | CTGCTGGAAAGACCACGAT | GACCACCAACATCCCATACA | 132 | 83.5 | 1.02 | 1.12 | 1.03 |
| LEA26 | – | – | Csa2G151040 | CGTTGACTTACCCATCACCTTC | GCGTGTAGTACCACCCTCTTTA | 163 | 85.5 | 1.00 | 1.06 | 0.98 |
| 40SRPS8 | – | – | Csa6G382970 | ACTCGACACTGGAAACTACTCG | CCTGAACAACGGCACTCTT | 134 | 85.1 | 0.87 | 1.03 | 1.01 |