| Ranking Order of candidate reference genes in different organs of cucumber plants (Better–Good–Average) |
| Delta CT |
TIP41 |
CACS |
40SRPS8 |
TUA |
PP2A |
CYP |
UBC |
RPL36Aa |
ACT |
ARF |
LEA26 |
F-Box |
| BestKeeper |
CACS |
TIP41 |
40SRPS8 |
PP2A |
TUA |
RPL36Aa |
CYP |
ARF |
F-Box |
ACT |
LEA26 |
UBC |
| Normfinder |
TIP41 |
CACS |
40SRPS8 |
PP2A |
TUA |
CYP |
ARF |
RPL36Aa |
UBC |
ACT |
LEA26 |
F-Box |
| geNorm |
TIP41 — 40SRPS8 |
CACS |
TUA |
PP2A |
CYP |
UBC |
ACT |
ARF |
LEA26 |
RPL36Aa |
F-Box |
| Recommended comprehensive ranking |
TIP41 |
CACS |
40SRPS8 |
PP2A |
TUA |
CYP |
RPL36Aa |
ARF |
UBC |
ACT |
LEA26 |
F-Box |
| Ranking Order of candidate reference genes in different organs of pumpkin plants (Better–Good–Average) |
| Delta CT |
TIP41 |
PP2A |
UBC |
F-Box |
ARF |
CACS |
CYP |
ACT |
40SRPS8 |
RPL36Aa |
LEA26 |
TUA |
| BestKeeper |
TIP41 |
UBC |
PP2A |
F-Box |
ARF |
CYP |
ACT |
CACS |
LEA26 |
40SRPS8 |
RPL36Aa |
TUA |
| Normfinder |
TIP41 |
PP2A |
UBC |
F-Box |
ARF |
CYP |
ACT |
CACS |
40SRPS8 |
RPL36Aa |
LEA26 |
TUA |
| geNorm |
CACS — 40SRPS8 |
RPL36Aa |
PP2A |
TIP41 |
UBC |
ACT |
CYP |
F-Box |
ARF |
LEA26 |
TUA |
| Recommended comprehensive ranking |
TIP41 |
PP2A |
UBC |
CACS |
F-Box |
40SRPS8 |
ARF |
CYP |
ACT |
RPL36Aa |
LEA26 |
TUA |
| Ranking Order of candidate reference genes in different organs of cucumber/pumpkin grafted plants (Better–Good–Average) |
| Delta CT |
CACS |
40SRPS8 |
ARF |
CYP |
TUA |
RPL36Aa |
UBC |
TIP41 |
LEA26 |
F-Box |
ACT |
PP2A |
| BestKeeper |
CYP |
RPL36Aa |
40SRPS8 |
ARF |
CACS |
LEA26 |
UBC |
TUA |
ACT |
TIP41 |
F-Box |
PP2A |
| Normfinder |
40SRPS8 |
CACS |
ARF |
TUA |
CYP |
RPL36Aa |
TIP41 |
F-Box |
UBC |
LEA26 |
ACT |
PP2A |
| geNorm |
CACS — ARF |
40SRPS8 |
CYP |
RPL36Aa |
TUA |
UBC |
LEA26 |
ACT |
TIP41 |
F-Box |
PP2A |
| Recommended comprehensive ranking |
CACS |
40SRPS8 |
ARF |
CYP |
RPL36Aa |
TUA |
UBC |
LEA26 |
TIP41 |
F-Box |
ACT |
PP2A |
| Ranking Order of candidate reference genes in graft union of cucumber/pumpkin plants under low temperature (Better–Good–Average) |
| Delta CT |
TUA |
CACS |
RPL36Aa |
F-Box |
40SRPS8 |
CYP |
ARF |
ACT |
UBC |
LEA26 |
PP2A |
TIP41 |
| BestKeeper |
TUA |
RPL36Aa |
CACS |
CYP |
40SRPS8 |
F-Box |
ACT |
ARF |
UBC |
PP2A |
TIP41 |
LEA26 |
| Normfinder |
TUA |
CACS |
RPL36Aa |
F-Box |
40SRPS8 |
ARF |
ACT |
CYP |
UBC |
LEA26 |
PP2A |
TIP41 |
| geNorm |
CYP — UBC |
40SRPS8 |
RPL36Aa |
TUA |
ACT |
CACS |
F-Box |
ARF |
LEA26 |
PP2A |
TIP41 |
| Recommended comprehensive ranking |
TUA |
RPL36Aa |
CACS |
CYP |
40SRPS8 |
UBC |
F-Box |
ACT |
ARF |
LEA26 |
PP2A |
TIP41 |
| Ranking Order of candidate reference genes in graft union during healing process (Better–Good–Average) |
| Delta CT |
LEA26 |
F-Box |
TIP41 |
40SRPS8 |
RPL36Aa |
ARF |
UBC |
CACS |
TUA |
ACT |
PP2A |
CYP |
| BestKeeper |
ARF |
TIP41 |
F-Box |
40SRPS8 |
RPL36Aa |
ACT |
LEA26 |
UBC |
CYP |
CACS |
TUA |
PP2A |
| Normfinder |
LEA26 |
F-Box |
40SRPS8 |
TIP41 |
RPL36Aa |
ARF |
UBC |
CACS |
TUA |
ACT |
PP2A |
CYP |
| geNorm |
UBC — ARF |
F-Box |
LEA26 |
TIP41 |
RPL36Aa |
40SRPS8 |
CACS |
TUA |
ACT |
PP2A |
CYP |
| Recommended comprehensive ranking |
LEA26 |
ARF |
F-Box |
TIP41 |
40SRPS8 |
UBC |
RPL36Aa |
CACS |
ACT |
TUA |
CYP |
PP2A |
| Ranking Order of candidate reference genes in graft union of different varities of grafted plants (Better–Good–Average) |
| Delta CT |
TIP41 |
PP2A |
UBC |
ARF |
40SRPS8 |
RPL36Aa |
LEA26 |
CACS |
ACT |
TUA |
F-Box |
CYP |
| BestKeeper |
TIP41 |
LEA26 |
PP2A |
ARF |
UBC |
RPL36Aa |
ACT |
40SRPS8 |
CACS |
F-Box |
TUA |
CYP |
| Normfinder |
TIP41 |
UBC |
PP2A |
40SRPS8 |
RPL36Aa |
ARF |
LEA26 |
ACT |
CACS |
TUA |
F-Box |
CYP |
| geNorm |
PP2A — ARF |
TIP41 |
40SRPS8 |
RPL36Aa |
UBC |
CACS |
LEA26 |
ACT |
F-Box |
TUA |
CYP |
| Recommended comprehensive ranking |
TIP41 |
PP2A |
ARF |
UBC |
40SRPS8 |
LEA26 |
RPL36Aa |
CACS |
ACT |
F-Box |
TUA |
CYP |
| Ranking Order of candidate reference genes in all samples in grafted cucumber/pumpkin plants (Better–Good–Average) |
| Delta CT |
CACS |
40SRPS8 |
LEA26 |
UBC |
ARF |
TUA |
F-Box |
ACT |
RPL36Aa |
TIP41 |
CYP |
PP2A |
| BestKeeper |
CACS |
LEA26 |
40SRPS8 |
TUA |
UBC |
RPL36Aa |
ARF |
ACT |
F-Box |
CYP |
TIP41 |
PP2A |
| Normfinder |
CACS |
40SRPS8 |
UBC |
TUA |
LEA26 |
ARF |
F-Box |
ACT |
RPL36Aa |
TIP41 |
CYP |
PP2A |
| geNorm |
ARF — 40SRPS8 |
CACS |
LEA26 |
TUA |
UBC |
ACT |
F-Box |
TIP41 |
RPL36Aa |
CYP |
PP2A |
| Recommended comprehensive ranking |
CACS |
40SRPS8 |
LEA26 |
ARF |
UBC |
TUA |
F-Box |
ACT |
RPL36Aa |
TIP41 |
CYP |
PP2A |
| Ranking Order of candidate reference genes in all samples (Better–Good–Average) |
| Delta CT |
CACS |
40SRPS8 |
ARF |
UBC |
TUA |
LEA26 |
F-Box |
ACT |
TIP41 |
RPL36Aa |
CYP |
PP2A |
| BestKeeper |
LEA26 |
CACS |
UBC |
TUA |
40SRPS8 |
RPL36Aa |
CYP |
ARF |
ACT |
TIP41 |
F-Box |
PP2A |
| Normfinder |
CACS |
40SRPS8 |
ARF |
TUA |
F-Box |
UBC |
LEA26 |
ACT |
TIP41 |
RPL36Aa |
CYP |
PP2A |
| geNorm |
CACS — 40SRPS8 |
ARF |
LEA26 |
UBC |
TUA |
ACT |
F-Box |
TIP41 |
RPL36Aa |
CYP |
PP2A |
| Recommended comprehensive ranking |
CACS |
40SRPS8 |
LEA26 |
ARF |
UBC |
TUA |
F-Box |
ACT |
RPL36Aa |
TIP41 |
CYP |
PP2A |
