Table 1.
Part list used to build the C. eth-1.0 genome design
| DNA part category | Quantity | Size (bp) | Fraction (%) |
| Protein-coding sequences | 676 | 660,789 | 83.9 |
| Essential | 462 | 471,072 | 59.8 |
| Semiessential | 113 | 114,270 | 14.5 |
| Redundant | 15 | 14,970 | 1.9 |
| Nonessential | 86 | 60,477 | 7.7 |
| Noncoding sequences | 54 | 9,726 | 1.2 |
| tRNA | 44 | 3,455 | 0.4 |
| rRNA | 3 | 4,387 | 0.6 |
| ncRNA | 7 | 1,884 | 0.2 |
| Intergenic sequences | 1,015 | 96,043 | 12.2 |
| Genome replication and assembly | 16 | 19,143 | 2.5 |
| Click markers* | 5 | 6,352 | 0.8 |
| ARS | 10 | 2,121 | 0.3 |
| pMR10Y† | 1 | 10,670 | 1.4 |
| Total no. of DNA parts | 1,761 | 785,701 |
*
Auxotrophic selection markers that were used to direct the assembly and maintenance of the genome design in yeast. ncRNA, noncoding RNA.
†
The pMR10Y shuttle vector contains a broad host-range RK2-based low copy replicon (GenBank accession no. AJ606312.1), a kanamycin selection marker, and oriT function for conjugational transfer from E. coli to Caulobacter as well as URA3 marker, ARS, and centromere (CEN) elements for selection and replication in yeast.