Skip to main content
. 2019 Apr 1;116(16):8010–8017. doi: 10.1073/pnas.1821844116

Fig. 4.

Fig. 4.

Transfer of the p.C328S/p.C343S mutated form of pejvakin into Pjvk−/− IHCs does not restore neither early pexophagy nor peroxisome proliferation after sound exposure. (A) The number of LC3B/pejvakin in situ PLA spots does not increase at 1 h after sound exposure (5–40 kHz, 105 dB SPL, 1 h) in Pjvk−/− IHCs transduced with AAV2/8-Pjvk(p.C328S;C343S)-IRES-EGFP (n = 40 IHCs; four mice per condition). (B) Peroxisome degradation (at 1 h after sound exposure) and proliferation (at 48 h after sound exposure) are not restored in Pjvk−/− IHCs transduced with AAV2/8-Pjvk(p.C328S;C343S)-IRES-EGFP (n = 60 IHCs; four mice per condition). Data are mean ± SEM. ***P < 0.001; n.s., not significant, unpaired Student’s t test. (Scale bar: 5 μm.)