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. 2019 Apr 2;116(16):7825–7830. doi: 10.1073/pnas.1816893116

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Fig. 7. — Functionality of mepK variants and orthologs. (A) ∆mepS ∆mepK strain carrying either vector (P_trc), pPK17 (P_trc::mepK), pPK19 (P_trc::mepK_H133A), pPK20 (P_trc::mepK_D140A), or pPK21 (P_trc::mepK_H173A) was grown in LB, and viability was tested on NA plates with IPTG (0.5 mM) at 30 °C. (B) ∆mepS ∆mepK strain carrying vector (P_trc), pPK17 (P_trc::mepK-His_E. _coli;Ec), pPK23 (P_trc::mepK-His_C. _{crescentus;Cc}), or pPK25 (P_trc::mepK-His_{K. pneumoniae;Kp}) was grown in LB and processed as described above.