Fig. 1.

SFF system for the CPA-free cryopreservation of cells. (A) Schematic illustration of the entire system. (B) Enlarged illustration of the inkjet head, which is fixed and constantly ejects droplets. The droplets were printed on grid points at intervals of 200 μm by programming the automatic stage. (C) Fluorescence image of 200-pL droplets printed on a glass substrate cooled with LN. To visualize the droplets, the culture medium was supplemented with 0.1 mM fluorescein. (D) Microscopic images of 200- and 40-pL droplets, observed at an angle of ∼45°.