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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Mol Ecol. 2018 Dec 10;28(6):1460–1475. doi: 10.1111/mec.14904

Figure 4.

Figure 4.

Identification and segregation of high+ RLG1a genotype. a. Median-standardized coverage (25bp windows) of high+ carriers IM_627c and IM15P_317d, showing amplification of Migut.N02091 and the Migut.N02090–2091 intergenic region. b. Quantile box plots for RLG1a copy number in selfed progeny of IM13P_627c segregating for 0 (n = 7), 1 (n = 20) or 2 (n = 12) high+ variants. RLG1a copy number was estimated as 2(Δ Ct), where ΔCt is the absolute difference between Ct mN2091q and Ct mG571q for an individual, averaged across two technical replicates.