. Author manuscript; available in PMC: 2019 Apr 20.

Published in final edited form as: J Am Chem Soc. 2018 Dec 28;141(1):262–271. doi: 10.1021/jacs.8b09311

Table 1.

Time constants and amplitudes from the bi-exponential decay of SE, average ESLs, and experimentally determined fluorescence QYs (Φ) with respect to that of bacteriorhodopsin (Φ_bR), determined from the spectrally integrated fluorescence intensities. See footnote ^a.

Sample	A1	t1 (ps)	A2	t2 (ps)	ESL (ps)	Φ/Φ_bR
DA L83Q	0.78	0.27±0.05	0.22	1.2±0.2	0.48	2.4
DA WT	0.72	0.55±0.05	0.28	1.7±0.2	0.86	1.1
OA W76S/Y179F	0.60	2.8±0.3	0.40	10±0.8	5.7	8.7
GA W76S/Y179F	0.40	2.1±0.4	0.60	8±0.7	5.7	7.6

^a.

The fluorescence QY’s are determined with bacteriorhodopsin as a reference sample (see SI). However, we refrain from quoting absolute values since the only available experimentally determined value for bacteriorhodopsin appears to be excessively high, most probably due to contributions from later photo-cycle intermediates.³⁴