Skip to main content
. Author manuscript; available in PMC: 2020 Jan 23.
Published in final edited form as: J Am Chem Soc. 2018 Dec 31;141(3):1251–1260. doi: 10.1021/jacs.8b10131

Figure 2. Favored local geometries for a T = 4 capsid or hexagonal repeat.

Figure 2.

(a) Gallery of negative stain TEM images. Particles in each vertical column were clustered together during a reference-free 2D class averaging procedure. Each column is a representative sample of a class with more particles. (b) The corresponding class averages sorted approximately by ascending size. Particles with a diameter of 35 nm, expected for normal T = 4 capsids, appear on the far left. Larger aberrant structures observed from assembly with HAP-TAMRA include larger, roughly spherical structures, ellipsoidal structures, and structures that appear to have shapes of tubes and spirals. (c) A common feature among all class averaged structures is the highlighted regions with a curvature of 35 nm diameter, comparable to that T = 4 capsids. Particles were assembled from 5 μM Cp dimer with 20 μM HAP-TAMRA and 300 mM NaCl in 50 mM HEPES (pH 7.5).