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. Author manuscript; available in PMC: 2020 Jan 23.
Published in final edited form as: J Am Chem Soc. 2018 Dec 31;141(3):1251–1260. doi: 10.1021/jacs.8b10131

Figure 5. Evolution of assembly reaction products over time.

Figure 5.

Histograms show the relative stability and evolution of particles assembled from 5 μM dimer with 20 μM HAP-TAMRA and 300 mM NaCl in 50 mM HEPES on the timescale of months and compared to a T = 4 capsid standard. The reaction solution was purified with size exclusion chromatography (SEC) to remove unassembled dimer and excess drug from the solution. Over time, the particles mature to larger sizes, and the distribution becomes more symmetrical. The number of measured particles is >900 for all histograms. For clarity, the y-values for the histograms in the presence of HAP-TAMRA have been multiplied by 4.