(A and B) Cell viability after 72 hours of exposure to CX-4945 (10 μΜ), assessed by luminescent cell viability assays, in murine Ptch+/− (MB21 and MB55), Ptch+/−;Tpr53−/− (MB53) MB lines in culture (A) and in human MB PDX lines (RCMB32, BT084, ICb-984, and ST01) assayed in culture (B). (C and D) Cell viability and cell cycle stage of human SHH MB cells cultured in DMSO, CX-4945, or vismodegib for 24 hours, as assessed by high-throughput single-cell imaging. (E) Cell viability after 72 hours of exposure to CX-4945 (10 μM), assessed by luminescent cell viability assays, in pediatric glioma (DIPG) cells. Data are means ± SD from n = 3 experiments.