Table 4.
Effects of neem (Azadirachta indica) oil on some biochemical and hematological parameters.
| Items | NOR | DMBA | Tamox + DMBA | Neem 1 + DMBA | Neem 2 + DMBA |
|---|---|---|---|---|---|
| Transaminases | |||||
| ALT | 63.5± 2.37 | 69.33± 2.86 | 65.2± 2.35 | 55.16± 3.28 | 79.1± 1.21∗ |
| AST | 45.5± 0.79 | 47.33± 1.28 | 49.6± 4.91 | 42.5± 1.85 | 61.2± 0.71∗∗∗ |
|
| |||||
| Hematological parameter | |||||
| WBC (×103μL−1) | 13.3± 0.66 | 8.33± 0.54 | 10.77± 1.67 | 8.37± 1.67 | 5.5± 0.17 |
| Lymphocytes (%) | 43.2± 0.66 | 50.43± 1.43 | 50.775± 2.33 | 56.45± 1.69 | 53.6± 0.45 |
| Monocytes (%) | 18.95± 0.67 | 19.4± 1.52 | 18.875± 1.41 | 18.075± 1.13 | 18.3± 0.28 |
| Granulocytes (%) | 37.85±1.34 | 30.16± 0.94 | 24.225±1.53 | 25.475± 2.21 | 27.85±0.44 |
| RBC (×103μL−1) | 5.57± 0.16 | 1.40± 0.03### | 1.21± 0.14 | 1.395 ± 0.07 | 1.07± 0.09∗∗ |
| Hematocrit (%) | 7.58± 0.66 | 7.36± 0.13 | 6.95± 0.93 | 6.55± 0.68 | 6.1± 0.28 |
| MCV (fL) | 173.7± 19.37 | 102.23± 2.42# | 147.56± 9.98 | 121.15±6.79 | 145.45± 5.55 |
| Platelets (×103μL−1) | 1007± 116.62 | 2721.5± 28.86### | 2419.75±72.64 | 1453.06± 84.64∗∗∗ | 1416.03± 10.69∗∗∗ |
| MCH (pg) | 7.5875± 0.66 | 7.36±0.13 | 6.95± 0.93 | 6.55± 0.68 | 6.1± 0.28 |
| Hemoglobin (g/dL) | 139.5± 6.20 | 143± 1.32 | 149± 3.31 | 137.25±3.00 | 154.75± 0.33 |
| MCHC (g/dL) | 2493± 222.73 | 1945± 35.79 | 3159.37± 122.81∗ | 2460.33± 324.42 | 2374±67.70 |
NOR-1: normal rats fed with SD treated with cotton oil; NOR-2: normal rats fed with HFHS treated with cotton oil; DMBA: negative control treated with cotton oil; TAM + DMBA: animals treated with tamoxifen (3.3 mg/kg); Neem + DMBA: animals treated with neem (Azadirachta indica) oil at a dose of 3 mL/kg at three different frequencies. All groups except the normal groups (NOR-1 and NOR-2) received subcutaneous dose of DMBA at the dose of 50 mg/kg. Data are represented as mean ± SEM (n = 5). #p < 0.05, ###p < 0.001 as compared to normal rats (NOR-2); ∗p < 0.05, ∗∗∗p < 0.001 as compared to DMBA.