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. 2019 Apr 18;7:e6789. doi: 10.7717/peerj.6789

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©2019 Albertini et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Sequence (2 min long) of light micrographs in vivo of a bud generated by an organism treated with EtOH. Sequence of light micrographs in vivo of a single sphere generated by an organism treated with EtOH. Pictures showing changes in the bud body shape, were taken at the 30th day, when the budding process was in progress. The entire process, from the first recognizable primordium of the small spheres to the detachment, took about 8 h.