KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals, Peptides, and Recombinant Proteins | ||
| Cell Tracker green | Life Technologies | Cat# C7025 |
| Cell Tracker red | Life Technologies | Cat# C34552 |
| Y-27632 | Tocris Bioscience | Cat# 1254 |
| (−)-tetramisole hydrochloride | Sigma-Aldrich | L9756 |
| Critical Commercial Assays | ||
| Cell Line Nucleofector Kit V | Lonza | Cat# VCA-1003 |
| In-Fusion cloning kit | Takara Bio | Cat# 638910 |
| Experimental Models: Cell Lines | ||
| MCF-7 | ATCC | HTB-22 |
| Experimental Models: Organisms/Strains | ||
| C. elegans: N2 | Caenorhabditis Genetics Center | N2 |
| C. elegans: him-4(e1267) X | Caenorhabditis Genetics Center | CB1267 |
| C. elegans: him-8 (e1489) IV | Caenorhabditis Genetics Center | CB1489 |
| C. elegans: act-2 (or621)V | Caenorhabditis Genetics Center | EU1295 |
| C. elegans: qIs56 V [Plag-2::GFP + unc-119(+)] | Caenorhabditis Genetics Center | JK2868 |
| C. elegans: syIs50 [Pcdh-3::GFP + dpy-20(+)] | Caenorhabditis Genetics Center | PS3352 |
| C. elegans: him-5(e1490) V; syIs78 [ajm-1::GFP + unc-119(+)] | Caenorhabditis Genetics Center | PS4657 |
| C. elegans: him-8 (e1489) IV; syIs50 [Pcdh-3::GFP + dpy-20(+)] | This paper | him-8- syIs50 |
| C. elegans: him-8 (e1489) IV; wimEx3 [Plag-2::hmp-2::DsRed + Plin-48::EGFP + pRF4(rol-6(su1006))] | This paper | him-8- wimEx3 |
| C. elegans: him-8 (e1489) IV; wimEx5 [Plag-2::GFP-UtrCH + pRF4(rol-6(su1006))] | This paper | him-8- wimEx5 |
| C. elegans: him-8 (e1489) IV; wimEx8 [Plin-48::lmp-1::DsRed + pRF4(rol-6(su1006))] | This paper | him-8- wimEx8 |
| C. elegans: him-8 (e1489) IV; wimEx30 [Plag-2::mCherry-UtrCH + Plin-48::PH::Venus + pRF4(rol-6(su1006))] | This paper | him-8- wimEx30 |
| C. elegans: him-8 (e1489) IV; wimEx52 [Plag-2::act-2::DsRed + Plin-48::EGFP + pRF4(rol-6(su1006))] | This paper | him-8- wimEx52 |
| C. elegans: him-8 (e1489) IV; syIs50 [Pcdh-3::GFP + dpy-20(+)], wimEx59 [Plag-2::act-2 (or621)::DsRed + pRF4(rol-6(su1006))] | This paper | him-8- syIs50- wimEx59 |
| C. elegans: him-8 (e1489) IV; wimEx63 [Plag-2::mCherry-UtrCH + Plin-48::EGFP + pRF4(rol-6(su1006))] | This paper | him-8- wimEx63 |
| C. elegans: him-8 (e1489) IV; wimEx74 [Plag-2::DsRed + pRF4(rol-6(su1006))] | This paper | him-8- wimEx74 |
| C. elegans: him-8 (e1489) IV; wimEx77 [Plag-2::tagBFP2 + Plin-48::DsRed + pRF4(rol-6(su1006))] | This paper | him-8- wimEx77 |
| C. elegans: him-8 (e1489) IV; wimEx83 [Plag-2::mCherry-UtrCH + Plag-2::NLS-GFP+ pRF4(rol-6(su1006))] | This paper | him-8- wimEx83 |
| C. elegans: him-4(e1267) X; wimEx83 [Plag-2::mCherry-UtrCH + Plag-2::NLS-GFP+ pRF4(rol-6(su1006))] | This paper | him-4- wimEx83 |
| C. elegans: him-5(e1490) V; syIs78 [ajm-1::GFP + unc-119(+)], wimEx77 [Plag-2::tagBFP2 + Plin-48::DsRed + pRF4(rol-6(su1006))] | This paper | syIs78- wimEx77 |
| Oligonucleotides | ||
| lin-48 promoter Forward primer for infusion: GGTTCCGCGTGGATCCCCTGCATTTTTTTCAGAGTCTATAATATCCGT | eurofins | N/A |
| lin-48 promoter Reverse primer for infusion: GCTCACCATGCGGCCGCCTGAAATTGAGCAGAGCTGAAAATTTTTGT | eurofins | N/A |
| lmp-1 forward primer: AAGCGGCCGCATGTTGAAATCGTTTGTCAT | eurofins | N/A |
| lmp-1 reverse primer: TTGCGGCCGCCGACGCTGGCATATCCTTGTC | eurofins | N/A |
| act-2 forward primer: AAATGATCGGCGGCCGCATGTGTGACGACGATGTTGCCGCTCTCGTA | eurofins | N/A |
| act-2 reverse primer: GCTCACCATGCGGCCGCCGAAGCATTTGCGATGAACAATTGAT | eurofins | N/A |
| hmp-2 forward primer for infusion: AAATGATCGGCGGCCGCATGCGATTATTCTCATATTTGGACG | eurofins | N/A |
| hmp-2 reverse primer for infusion: GCTCACCATGCGGCCGCCCAAATCGGTATCGTACCAATTGTGA | eurofins | N/A |
| Recombinant DNA | ||
| pBabe–H2B–mCherry | Joan Brugge Lab | N/A |
| pQCXIP-GFP | Xuejun Jiang Lab | N/A |
| GFP–C1–PLCdelta-PH | Stauffer et al., 1998 | Addgene Plasmid #21179 |
| pFx_Plag-2::venusT | Shohei Mitani Lab | N/A |
| pPK699 | Patricia Kuwabara Lab | N/A |
| GFP-UtrCH | Burkel et al., 2007 | Addgene plasmid #26737 |
| mCherry–UtrCH | Burkel et al., 2007 | Addgene plasmid #26740 |
| pRF4 (rol-6(su1006)) | Cole Haynes Lab | N/A |
| pBluescript | Cole Haynes Lab | N/A |
| Plin-48::EGFP | This paper | Plasmid #113 |
| Plag-2::hmp-2::DsRed | This paper | Plasmid #43 |
| Plag-2::GFP-UtrCH | This paper | Plasmid #35 |
| Plin-48::lmp-1::DsRed | This paper | Plasmid #42 |
| Plag-2::mCherry-UtrCH | This paper | Plasmid #36 |
| Plin-48::PH::Venus | This paper | Plasmid #51 |
| Plag-2::act-2::DsRed | This paper | Plasmid #68 |
| Plag-2::act-2 (or621)::DsRed | This paper | Plasmid #70 |
| Plag-2::DsRed | This paper | Plasmid #23 |
| Plag-2::tagBFP2 | This paper | Plasmid #123 |
| Plin-48::DsRed | This paper | Plasmid #114 |
| Plag-2::NLS-GFP | This paper | Plasmid #127 |
| Software and Algorithms | ||
| NIS Elements software | Nikon | N/A |
| Volocity image analysis software | Perkin Elmer | N/A |
| ImageJ | Schneider et al., 2012 | N/A |
| Other | ||
| 35 mm glass-bottom dishes | MatTek | Cat# P06G-1.5-20-F |
| Kwik-Fil borosilicate glass capillary | World Precision Instruments, Inc. | Cat# 1B100F-4 |
| 24×60 mm No. 1.5 cover glass | Fisherbrand | 12-545M |
| 0.25 mm-thick clear silicone sheet | Electron Microscopy Sciences | Cat# 70338-29 |