Figure 1.
Ng expression in the brain regulates PPI. A, There are no differences in startle response between Ng+/+ mice and Ng −/− mice. B, Ng −/− mice show a significantly decreased PPI compared to Ng+/+ mice. (n = 8 ~ 14; C57BL/6J x129S1 cross). C, Western blot analysis of the expression of oNg in the HEK293T cells (infected) and control (uninfected) cell. Startle changes in response to brain-specific Ng expression in C57BL/6J mice. D, Ng expression in the NAc does not induce changes in PPI. E, Overexpression of Ng (oNg) in the mPFC significantly increases PPI compared to that of dominant-negative Ng (dnNg) expression. F, Ng expression in the hippocampus does not induce changes in PPI. Control indicates baseline startle and PPI of C57BL/6J mice without stereotaxic surgery. Statistical significance was measured by Two-way ANOVA followed by Tukey’s test. (n = 8 per group). Data presented as mean ± SEM.