Fig. 3.

Cell-to-cell allelic imbalance of MYH7-mRNA in three HCM patients. Individual cells were isolated from sections of cardiac tissue via laser capture microdissection. Cells were lysed and the MYH7-mRNA was amplified by single cell RT-PCR. The fractions of mutant vs. wildtype transcript were determined by densitometric analysis of allele-specific restriction digests. Depicted are the fractions of mutant MYH7-mRNA in individual cardiomyocytes from three different HCM-patients (R723G-1, R723G-2, and A200V). Each dot represents one cardiomyocyte