Subject Area:	Agricultural and Biological Sciences
More specific subject area:	Natural product isolation Analytical glycobiology
Method name:	Improved strategy for large scale isolation and purification of sialylglycopeptide from egg yolk powder
Name and reference of original method:	This method was significantly modified for isolating the glycosylated hexapeptides as described by Seko et al. ‘Occurence of a sialylglycopeptide and free sialylglycans in hen's egg yolk’. Biochim Biophys Acta. 1997 Apr 17;1335(1-2):23-32. The herein described improved method uses the chloroform/methanol method described by Wessel, D. and U.I. Flugge, ‘A Method for the Quantitative Recovery of Protein in Dilute-Solution in the Presence of Detergents and Lipids’. Analytical Biochemistry, 1984. 138(1): p. 141-143., as a replacement for the phenol extraction used by Seko et al. for isolation of the desired glycosylated hexapeptide from egg yolk powder.
Resource availability:	N/A