Figure 1.

High-Resolution TEM Images of AlgS-Ca²⁺-pDNA NPs

(A and B) Dry-TEM micrographs of NPs (2.5 μg/mL AlgS, 25 mM Ca²⁺, and 15 ng/μL pDNA) with gold-labeled AlgS. (C) Cryo-TEM micrographs of complexes (250 mM Ca²⁺ and 150 ng/μL pDNA). (D) Cryo-TEM micrographs of NPs (25 μg/mL AlgS, 250 mM Ca²⁺, and 150 ng/μL pDNA). Scale bars, 500 nm (A) and 100 nm (B–D).