Fig. 1.

Brd4 was up-regulated in the kidney after mice were suffered from IRI. (A) Brd4 protein levels were detected by western blotting analysis at various reperfusion time points, such as 6 h, 12 h, and 24 h, and bar graph showing the fold changes of Brd4 relative to sham group from three independent samples. (B) Brd4 mRNA levels were detected by real-time RT-PCR at 6 h, 12 h, 24 h of reperfusion time. (C–D) Bar graph showing the fold changes of Brd4 positive areas relative to sham group, and immunohistochemical staining of Brd4 in renal tissues at 6 h, 12 h, 24 h of reperfusion time. Values are expressed as the mean ± SEM. *P < 0.05, relative to the sham group, n = 3.