JQ1 treatment protected kidney against I/R. (A) Western blots of Brd4 protein expression after treatment of mice with JQ1 at doses of 25 mg/kg, 50 mg/kg, 100 mg/kg, and bar graph showing the fold changes of Brd4 relative to sham group from three independent samples. (B–C) Protective effect of JQ1 at doses of 25 mg/kg, 50 mg/kg, 100 mg/kg on renal function of mice exposed to renal IRI. (D–E) Protective effect of JQ1 at various doses of 25 mg/kg, 50 mg/kg, 100 mg/kg on renal tissue damage detected by H&E (×400), and randomly selected image fields from eight independent kidney samples were used for quantification renal tubular injury scores. Values are expressed as the mean ± SEM. *P < 0.05, relative to sham group; **P < 0.05, relative to vehicle control; #P < 0.05, relative to I/R+JQ1 (25 mg/kg); & P < 0.05, relative to I/R+JQ1 (50 mg/kg).