JQ1 attenuated FoxO4-mediated oxidative stress via the PI3K/AKT pathway in mice. (A–C) MDA, SOD and H2O2 production in mice subjected to I/R with or without JQ1. (D) Western blot analysis for the protein expression of FoxO4 in mice treated with or without JQ1 at 24 h of reperfusion time and quantification. (E–F) Western blot analysis for the protein expression of PI3K, p-PI3K, AKT, p-AKT with or without JQ1 at 24 h of reperfusion time and quantification. *P < 0.05 versus Sham, #P < 0.05 versus I/R.