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. 2019 Apr 17;93(9):e02036-18. doi: 10.1128/JVI.02036-18

TABLE 2.

Primersa

Target Forward primer (5′–3′) HBV positions (genotype C) Reverse primer (5′–3′) HBV positions (genotype C)
Analysis of ChIP samples
    Actin TSS TAG AAG TCG CAG GAC CAC ACT NA TGG GTA GGT TTG TAG CCT TCA T NA
    Nanog TSS TGG GTT TGT CTT CAG GTT CTG NA CTA CTG ACC CAC CCT TGT GAA NA
    Sat2 CAT CGA ATG GAA ATG AAA GGA GTC NA ACC ATT GGA TGA TTG CAG TCA A NA
    HBV_1 TTA ACA GGC CTA TTG ATT GGA AA 965–984 TCA ACG CAG GAT AAC CAC ATT 1034–1049
DNA quantification
    HBV-DNA CCA AAA TTC GCA GTC CCC AAC 304–324 GAG GCA TAG CAG CAG GAT GAA G 406–427
RNA quantification
    pgRNA GAG TGT GGA TTC GCA CTC CTC 2268–2288 CGA GGC GAG GGA GTT CTT CT 2378–2397
    pg/S-RNA CCC GTT TGT CCT CTA ATT CC 467–486 GTC CGA AGG TTT GGT ACA GC 567–585
    HBV-RNA GTG CAC TTC GCT TCA CCT CT 1579–1598 TTG ACA TTG CTG AGA GTC CAA 1666–1686
    IMAP TTT TCA GCT CCC AAG TGT CC NA GCC GAG AGC AGG TAG CAG T NA
a

HBV primer positions are according to a well-annotated HBV genome (GenBank accession number AM282986) of genotype A (56). IMAP, immunity-associated protein; TSS, transcriptional start site; NA, not applicable.