FIG 7.

Analysis of ICP4 and LAT expression in HSV-1 acutely infected 10-week-old organoids at 48 h postinfection. (Top) Immunostaining of two paraffin-embedded sections of the same organoid infected with the HSV-1 immediate early gene ICP4. (Middle) Coimmunostaining of a HSV-1-infected organoid with ICP4 and the neuronal marker MAP2. (Bottom) Analysis of LAT expression by RNAscope in situ hybridization analysis. Nuclei were counterstained with Hoechst 33342. Bars, 250 μm (top) and 50 μm (middle).