FIG 1.

Alignment of NPB, NPC, and NPD sequences with reference to NPA shows that nuclear localization signals on NPA and NPB (as indicated by boxes) are absent at the corresponding positions on NPC. Sequences were aligned using ClustalO (50) and visualized by Esprit 3.0 (51). Two partially overlapping sequences on NPC were predicted as potential NLS sequences by NLStradamus and cNLSMapper servers, which are indicated by red and purple lines, respectively. Point mutations or deletions were then made on these two sequences, and the subcellular localization of variants was investigated.