FIG 3.

Us11 is not required for growth in vitro. HCLEs, either left untreated or pretreated with 30 IU/ml IFN-β (A), or U373s (B) were infected with the indicated viruses at MOI = 0.001. At 1 h postadsorption, viral inocula were replaced with media and wells (media and cells) harvested at the indicated time points and titers were determined on Vero cells. Data points represent means ± standard deviations (SD) of results from experiments performed three times. P = <0.01 to 0.0001 (two-way ANOVA) where shown. LOD, limit of detection.