FIG 1.

CD4⁺ and CD8⁺ T-cell responses against HSV-2 antigens detected from symptomatic and asymptomatic patients. (A) Average intensity of recognition of ORFs expressing HSV-2 proteins by T cells from HSV-2-seropositive symptomatic (n = 10) and asymptomatic individuals (n = 10), as tested by IFN-γ production. The names of HSV-2 ORFs driving two representative positive responses are indicated. (B) VP11/12- and VP13/14-specific CFSE⁺ proliferative T cells from symptomatic and asymptomatic individuals represented in panel A. (C and D) Representative analyses of HSV-2 VP11/12- and VP13/14-specific IFN-γ-producing CD4⁺ T cells (C) and CD8⁺ T cells (D). The top portion shows representative analysis from the one symptomatic and one asymptomatic individual, and the bottom portion shows averages from the 10 symptomatic and 10 asymptomatic individuals. Representative results are from three independent experiments. The P value compares the results from 10 symptomatic and 10 asymptomatic individuals. Values were calculated using the ANOVA two-tail test.