KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-digoxigenin-POD, Fab fragments | Roche | Cat# 11 207 733 910; RRID: AB_514500 |
| anti-fluorescein-POD, Fab fragments | Roche | Cat# 11 426 346 910; RRID: AB_840257 |
| anti-DNP-HRP conjugate | Perkin-Elmer | Cat# FP1129; RRID: AB_2629439 |
| Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8 | Millipore | Cat# 05-817R; RRID:AB_11215621 |
| Goat anti-rabbit antibody | ThermoFisher | Cat# 65-6120; RRID:AB_2533967 |
| Chemicals | ||
| TRIzol | Life Technologies | Cat#15596018 |
| Western Blocking Reagent | Roche | Cat#11921673001 |
| Critical Commercial Assays | ||
| ApopTag Red In Situ Apoptosis Detection Kit | Millipore | Cat# S7165 |
| Deposited Data | ||
| follistatin RNAi RNaseq | Scimone et al., 2017 | GEO: GSE99067 |
| Smed_dd_v6 transcriptome | Brandl et al., 2016 | http://planmine.mpi-cbg.de/planmine/begin.do |
| Experimental Models: Organisms/Strains | ||
| Schmidtea mediterranea, clonal strain CIW4, asexual | Laboratory of Peter Reddien | N/A |
| Oligonucleotides | ||
| Sequences used for all FISH probes and dsRNA provided in Table S1 | N/A | N/A |
| Primers used for qRT-PCR are provided in Table S2 | N/A | N/A |
| Software and Algorithms | ||
| ImageJ (FIJI) | Schindelin et al., 2012 | https://fiji.sc |
| ZEN digital imaging software | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| GraphPad Prism | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| R v.3.2.3 | The R Foundation | https://www.r-project.org/ |
| bowtie v1.1.2 | Langmead et al., 2009 | http://bowtie-bio.sourceforge.net/index.shtml |
| DESeq | Anders and Huber, 2010 | https://bioconductor.org/packages/release/bioc/html/DESeq.html |