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. Author manuscript; available in PMC: 2019 Apr 22.
Published in final edited form as: Nat Immunol. 2018 Mar 12;19(4):397–406. doi: 10.1038/s41590-018-0065-7

Fig. 4 |. Interaction between the 3C8 TCR and CD1c.

Fig. 4 |

a–d, Enlarged view of the interactions between residues of the 3C8 TCR CDR3α loop (a), CDR1α loop (b), CDR2α loop (c) and CDR3β loop (d) and the CD1c α-helices. Spheres indicate glycine side chains; red dotted lines indicate salt-bridge interactions; black dotted lines indicate hydrogen-bond interactions. e, Expression of CD69 by J76.3C8 cells after co-culture with C1R cells expressing wild-type CD1c (CD1c WT) or various CD1c alanine-substitution mutants (horizontal axis), or parental C1R cells, C1R.CD1d cells or no C1R cells (right end); results were normalized to those of C1R cells expressing wild-type CD1c, set as 100%, and dashed horizontal lines indicate a 25% or 50% reduction (as marked), relative to that expression (top). Below, the CD1c surface, showing the side-chain residues; colors indicate effect on activation (red, > 50% decrease; orange, 25–50% decrease; blue, < 25% decrease). Data represent two independent experiments, each in duplicate (e).