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. 2019 Apr 8;2019:8589149. doi: 10.1155/2019/8589149

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Copyright © 2019 Pascal Gervois et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Workflow to isolate L-PRF subfractions. The L-PRF clot is isolated by centrifugation from blood samples that are subsequently processed for CM L-PRF and EX L-PRF isolation. L-PRF clots are transferred to 6-well plates in culture medium, and CM L-PRF was collected after 96 h. The L-PRF clot is transferred to the Xpression™ Box and by gravitational compression of the clot, EX L-PRF is collected. Both CM- and EX L-PRF were filtered before use. This figure was partly created using Servier Medical Art licensed under a Creative Common Attribution 3.0 Generic License, available online at http://smart.servier.com/.