Skip to main content
. 2019 Apr 8;2019:8589149. doi: 10.1155/2019/8589149

Figure 3.

Figure 3

Metabolic and proliferative activity of hDPSCs exposed to CM and EX L-PRF and the effect of L-PRF priming on hDPSC-derived BDNF secretion. The metabolic (a, c) and proliferative (b, d) effect of hDPSCs that were exposed for 24 h, 48 h, and 72 h to CM (a, b) or EX L-PRF (c, d) was evaluated by means of an MTT and PI assay, respectively (n = 5). CM and EX L-PRF had an inverse effect on hDPSC metabolism as high concentrations of CM significantly diminished, and high concentrations of EX L-PRF significantly stimulated hDPSC metabolism. Both CM and EX L-PRF had a dose-response effect on proliferation as higher concentrations significantly enhanced hDPSC proliferation. BDNF secretion (e) was increased in hDPSCs primed (n = 4) with 10% CM L-PRF or 5% and 10% EX L-PRF for 24 h while significant BDNF secretion could only be observed when hDPSCs were primed for 48 h with 10% CM or EX L-PRF. ∗p value ≤ 0.05, ∗∗p value ≤ 0.01, and ∗∗∗p value ≤ 0.001. Data are expressed as mean ± SEM.