Table 1.
Primary and secondary antibodies for immunocytochemical analysis.
| Marker | Species | Clone, Cat no. | Dilution | Label | Company |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| A2B5 | MM | MAB312R | 1/200 | Millipore, Billerica, MA, USA | |
| BLBP | RP | ABN14 | 1/250 | Millipore | |
| β-III tubulin | RP | ab18207 | 1/50 | Abcam, Cambridge, UK | |
| β-III tubulin | MM | 2G10 | 1/2000 | Sigma-Aldrich | |
| CD45 | RP | Ab63390 | 1/100 | Abcam | |
| GFAP | MM | Clone G-A-5 | 1/400 | Sigma-Aldrich | |
| NCAM | RP | Ab5032 | 1/100 | Millipore | |
| NeuN | MM | A60, MAB377 | 1/100 | Millipore | |
| Sca-1 | GP | AF1226 | 1/50 | R&D, Minneapolis, MN, USA | |
| Sox2 | RP | Ab97959 | 1/1000 | Abcam | |
| Secondary antibodies | |||||
| DAM | DP IgG | A31570 | 1/500 | Alexa Fluor 555 | Invitrogen, Carlsbad, CA, USA |
| DAR | DP IgG | A21206 | 1/500 | Alexa Fluor 488 | Invitrogen |
| DAG | DP IgG | A11055 | 1/500 | Alexa Fluor 488 | Invitrogen |
BLBP: brain lipid-binding protein; GFAP: glial fibrillary acidic protein; NCAM: neural cell adhesion molecule; NeuN: neuronal nuclei; Sca-1: stem cell antigen-1; MM: mouse monoclonal antibody; RP: rabbit polyclonal antibody; GP: goat polyclonal; DP: donkey polyclonal; DAM: donkey anti-mouse; DAR: donkey anti-rabbit; DAG: donkey anti-goat.