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. Author manuscript; available in PMC: 2019 Apr 22.
Published in final edited form as: Curr Cancer Drug Targets. 2017;17(7):669–680. doi: 10.2174/1568009617666170315162932

Fig. (2).

Fig. (2).

Resveratrol-induced ROS mediates the apoptotic response. (A) Subconfluent (90%) monolayers of H460 cells were treated with varying doses of resveratrol in the presence and absence of NAC (10 mM) and MnTBAP (100 μM) for 1 hour. Superoxide levels were analyzed by spectrofluorometric measurement of DHE fluorescence. (B) Subconfluent (90%) monolayers of H460 cells were treated with varying doses of resveratrol in the presence and absence of NAC (10 mM) and Catalase 1000 (U/μl), for 1 hour. Hydrogen peroxide levels were analyzed by spectrofluorometric measurement of DCF fluorescence. (C) Cells were pretreated with MnTBAP (100 μM), Catalase (1000 U/μl) or NAC) (10 mM) for 1 hour followed by resveratrol (100 μM) treatment for 12 hours and analyzed for apoptosis by Hoechst 33342 assay. Graphs represent relative fluorescence intensity over untreated control. Data are mean ± S.E.M (n≤4). *, p < 0.05 versus non-treated control. #, p < 0.05 versus resveratrol (100 μM) treatment.