REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-p44/42 MAPK (Erk1/2) (137F5)	Cell Signaling Technology	Cat#4695; RRID: AB_390779
Rabbit anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)	Cell Signaling Technology	Cat#9101; RRID: AB_331646
Rabbit-Anti-Vinculin [EPR8185]	Abcam	Cat#ab129002; RRID: AB_11144129
Goat anti-rabbit IgG-HRP	Dako	Cat#P0448; RRID: AB_2617138
Mouse monoclonal anti-FLAG (M2) antibody	Sigma-Aldrich	Cat#F1804; RRID: AB_262044
Goat anti-mouse IgG-HRP	Dako	Cat#P0447; RRID: AB_2617137
Phycoerythrin (PE)-conjugated anti-DYKDDDDK tag antibody, clone L5	Biolegend	Cat#637310; RRID: AB_2563148
Bacterial and Virus Strains
XL10-Gold	Agilent	Cat# 200315
Chemicals, Peptides, and Recombinant Proteins
NDP-αMSH	Bachem	Cat#H-1100
α-MSH	Bachem	Cat#H-1075
β-MSH	Bachem	Cat#H-1475
Forskolin	Sigma-Aldrich	Cat#F6886
Recombinant human epidermal growth factor (EGF)	Invitrogen	Cat#PHG0311
Lipofectamine 2000™	GIBCO	Cat#11668
Critical Commercial Assays
GloSensor cAMP biosensor - pGloSensor 20F plasmid	Promega	Cat#E1171
GloSensor cAMP Reagent	Promega	Cat#E1290
NanoBiT protein:protein interaction assay	Promega	Cat#M2014
Nano-Glo Live Cell Assay System	Promega	Cat#M2013
QuickChange II XL kit	Agilent Technologies	Cat#200516
Experimental Models: Cell Lines
HEK293	ATCC	CRL-1573; RRID: CVCL_0045
HeLa	Provided by Kevin Moreau (University of Cambridge)	NA
Recombinant DNA
Human N-FLAG-MC4R-WT in pcDNA3.1(+) vector	Farooqi et al., 2000	NA
Human N-FLAG-MC4R variants in pcDNA3.1(+) vector	This paper	NA
Software and Algorithms
STATA v14.2	StataCorp	https://www.stata.com/stata14/
R v3.2.2	The R Foundation for Statistical Computing	https://cran.r-project.org/bin/windows/base/old/3.2.2/
BOLT-LMM v2.3.2	Loh et al., 2015	https://data.broadinstitute.org/alkesgroup/BOLT-LMM/
FIJI	Schindelin et al., 2012	https://fiji.sc
FlowJo	Tree Star	https://www.flowjo.com
Prism 7	Graph Pad Software	https://www.graphpad.com/scientific-software/prism/