Parameter	Meaning	Values	Units	Source
kL+	Ligand binding rate	4E+7	M-1s-1	Fitted
kL-	Ligand unbinding rate	3.10E-01	s-1	(Bridge et al., 2010)
kact+	Receptor activation rate to R∗	1.00E+00	s-1	“
kact-	Receptor deactivation rate from R∗	1.00E+03	s-1	“
kG+	G protein binding rate	1.00E+08	M-1s-1	“
kG-	G protein unbinding rate	1.00E-01	s-1	“
kGRA+	G protein re-association rate	7.00E+08	M-1s-1	Fitted
kGRA-	G protein dissociation rate	1.30E-03	s-1	(Bridge et al., 2010)
khyd+	Hydrolysis rate of GαGTP	1.00E-04	s-1	“
khyd-	Exchange rate of GTP to GDP at Gα	1.00E-04	s-1	“
kGTP+	R∗G dissociation rate	1.00E-02	s-1	Fitted
ν+	Forward cooperativity factor for ligand binding a G bound receptor	1.00E+00		(Bridge et al., 2010)
ν-	Backward cooperativity factor for ligand binding	1.00E+00		“
ζ+	Forward cooperativity factor for ligand-bound R activation	1.00E+03		“
ζ-	Backward cooperativity factor for ligand bound R activation	1.00E+00		“
μ+	Forward cooperativity factor for G bound R activation	1.00E+00		“
μ-	Backward cooperativity factor for G bound R activation	1.00E+00		“
kconact	Constitutive activity of Gβɣ	1.00E-10	s-1	Fitted
krev	Reverse reaction of constitutive activity in Gβɣ	1.00E-10	s-1	“
kE+1	STE5 binding rate to Gβɣ∗	1.00E+3	M-1s-1	“
kE+2	STE5 binding rate to Gβɣ	1.00E+9	M-1s-1	“
kE-1	STE5 dissociation rate to Gβɣ∗	1.00E+0	s-1	“
kE-2	STE5 dissociation rate to Gβɣ	1.00E+0	s-1	“
Rtot	Total receptor concentration	4.15E-10	M	“
Gtot	Total G protein concentration	4.15E-10	M	“
Ltot	Total ligand concentration	1.00E-04	M	“