Figure 3.

Changed gene expression of epigenetic effectors upon (A) β-glucan (1 μg/ml) or (B) oS100A4 (2 μg/ml), as revealed by RNASeq (box and whisker plots, significantly increased mean and median > 2-fold, n = 5 healthy donor in duplicate). (C) Heat map of epigenetic effectors transcripts discriminating the three conditions (controls [Co], β-glucan [βG] or oS100A4). (D) Increases in the cytoplasmic expression of selected epigenetic effectors in permeabilized monocytes upon oS100A4, as measured by flow cytometry (n = 6 healthy donors, Wilcoxon signed-rank test). The clustering was unsupervised. For each donor and transcript, a Z-score was calculated. Regarding a given transcript, the highest and lowest Z-scores are shown in green and red, respectively.