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. 2019 Apr 19;7:e6722. doi: 10.7717/peerj.6722

Table 2. Percentage (%) of individual fatty acids to total FA concentration of cultures in which: (i) O. marina was depleted of prey at the start of the experiments (Day 0 Om); (ii) the E. huxleyi cultures fed to O. marina; (iii) after three days fed non-infected E. huxleyi (Om + Eh non-inf); and (iv) after 3 days fed infected E. huxleyi (Om +Eh inf).

Values are mean ± one standard deviation, n = 4, n = 4, n = 3 and n = 3, respectively. Note the values for E. huxleyi are the average of duplicate non-infected and duplicate infected cultures.

FA Class Day 0 Om E. huxleyi Day 3 Om + Eh non-inf Day 3 Om + Eh inf
SFA C14:0 0.2 ± 0.2 0.2 ± 0.1 0.4 ± 0.1 1.2 ± 1.3
C15:0 0.4 ± 0.3 0.3 ± 0.2 0.5 ± 0.0 0.8 ± 0.4
C16:0 30.9 ± 4.5 27.6 ± 3.6 29.4 ± 7.6 24.6 ± 2.6
C17:0 2.7 ± 0.1 2.7 ± 0.7 2.7 ± 0.3 3.2 ± 0.1c,d
C18:0 36.2 ± 11.1 60.9 ± 7.6a 46.9 ± 6.4 56.4 ± 7.1c
C20:0 1.1 ± 0.1 1.3 ± 0.3 1.5 ± 0.8 1.2 ± 0.4
C22:0 0.8 ± 0.2 0.7 ± 0.6 3.3 ± 2.3 1.8 ± 0.9
C24:0 0.7 ± 0.5 1.1 ± 0.1 0.6 ± 0.4 0.4 ± 0.3
MUFA C16:1 0.8 ± 1.6 0.0 0.0 0.0
C18:1(n-9cis) 5.0 ± 4.0 0.0a 1.6 ± 1.4b 1.6 ± 1.6c
C18:1(n-9trans) 4.6 ± 3.3 0.0a 1.7 ± 1.5 1.3 ± 1.2
C22:1 1.1 ± 0.9 0.1 ± 0.1 0.0 0.0
PUFA C18:2 2.7 ± 1.4 0.0 0.0 b 0.2 ± 0.3c
C20:2 2.9 ± 2.3 5.0 ± 3.7 7.1 ± 1.9b 3.2 ± 1.3c,d
C20:5 (n–3) 1.2 ± 1.2 0.1 ± 0.1 0.2 ± 0.3 0.3 ± 0.3
C22:6 (n-3) 8.6 ± 7.7 0.0 4.0 ± 4.1 3.9 ± 3.3
∑ SFA 73 ± 15 95 ± 4a 86 ± 9 89 ± 6
∑ MUFA 12 ± 8 0.1 ± 0.1a 3 ± 3 3 ± 3
∑ PUFA 15 ± 8 5 ± 4 11 ± 6 8 ± 3

Notes.

Significant differences in the proportions of individual compounds are shown as:

a
Day 0 Om vs. E.huxleyi
b
Day 0 Om vs. Day 3 Om + Eh non-inf
c
Day 0 Om vs. Day 3 Om + Eh inf
d
Day 0 Om vs. Day 3 Om + Eh non-inf vs. Day 0 Om vs. Day 3 Om + Eh inf