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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: DNA Repair (Amst). 2019 Feb 27;77:10–17. doi: 10.1016/j.dnarep.2019.02.010

Figure 5. WA downregulates FANCA post-translationally through HSP90 inhibition and in a proteasome-dependent manner.

Figure 5.

(A) RT-PCR reveals that transcription of FANCA is elevated with 1.6 μM of WA treatment. (B) Normal half-life of FANCA with 5 μg/mL cycloheximide (CHX). (C) WA-mediated FANCA destabilization (at the indicated concentration for 4 hours) is blocked with proteasomal inhibition by MG132 (10 μM, starts 4 hours prior to WA addition). (D) WA impedes the interaction between FANCA and FISP90. Another FISP90 inhibitor AUY922 (at 0.1 μM) also disrupts the interaction in a time-dependent manner. (E) Combinatorial use of WA (1.6 μM) and AUY922 (0.1 μM) for 1.5 hour results in additive destabilization.