Figure 1. Standard curves of plate-based tau ELISAs and ultra-sensitive tau Simoa assays.
(A) Schematic representation of human tau441 and epitopes of the relevant antibodies. (B) Mid-region (BT2-Tau5), (C) NT1 (Tau12-BT2), (D) NT2 (Tau12-ADx202) and (E) full-length (Tau12-TauAB) ELISAs readily detect human tau with LLoQs ~30 pg/ml. Simoa-based (F) mid-region (BT2-Tau5) (LLoQ ~3.70 pg/ml), and Simoa-based (G) NT1 (BT2-Tau12), (H) NT2 (ADx202-Tau12), and full-length (TauAB-Tau12) assays are shown (LLoQ ~0.27 pg/ml). Each data point is the average ±SEM of three technical replicates. Where the error bars are not visible, the SEM is smaller than the size of the symbol.