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. 2019 Jul;30(2):19–24. doi: 10.7171/jbt.19-3002-001

FIGURE 1.

FIGURE 1

The effect of trypsin/EDTA Pretreatment on Ebola VP40 expression in a CACO-2 cell layer. A) Western immunoblot showing the levels of VP40 expression in CACO-2 cell layers transfected with various reagents/conditions as described in Materials and Methods. The conditions utilized were A) Lipofectamine, B) Lipofectamine 3000 (low formulation), C) Lipofectamine 3000 (high formulation), D) trypsin/EDTA pretreatment + Lipofectamine 3000 (low), and E) trypsin/EDTA pretreatment + Lipofectamine 3000 (high). B) VP40 band densitometric quantitation representing the means of 4 separate cell layers ± se. One-way ANOVA test. *P < 0.05, ***P < 0.001 vs. Lipofectamine-transfected cell layer.