FIGURE 2.
Effect of trypsin/EDTA pretreatment on CACO-2 barrier function following transfection. Cell layers were treated as described in Materials and Methods in mock transfections without the use of plasmid DNA. Cell Layers were treated with control medium, medium containing Lipofectamine, or Lipofectamine after a trypsin/EDTA pretreatment. Rt (A) and 14C-mannitol flux rate (B) were measured immediately after a 3-h transfection period. Data shown represent the percent of control resistance and mannitol flux rate, expressed as the means ± se of 6 cell layers per condition. One-way ANOVA test. *P < 0.05, ***P < 0.001.