Table 2. Genetic diversity for mtDNA regions, nucDNA regions for all ingroup sequences and the two major Scriptus and Sylvaticus clades.

	Locus	n	Size (bp)	S	π	h	Hd	k	S/k
Entire species complex	12SrRNA	27	593	63	0.036	21	0.98	21.348	2.951
	16SrRNA	27	347	35	0.038	17	0.954	13.137	2.664
	Cytochromeb	27	1,140	255	0.072	24	0.991	82	3.11
	MGF	27	671	10	0.003	5	0.635	1.852	5.399
	PRCK1	27	498	2	0.0003	3	0.145	0.148	13.51
	SPTBN1	27	764	12	0.001	7	0.456	0.957	12.539
	THY	27	663	2	0.0008	3	0.501	0.541	3.696
Scriptus clade	12SrRNA	27	593	17	0.012	8	0.972	7.167	2.371
	16SrRNA	27	347	3	0.003	3	0.667	1	3
	Cytochromeb	27	1,140	90	0.028	8	0.972	32.389	2.778
	MGF	27	671	0	0	1	0	0	2.712
	PRCK1	27	498	2	0.001	3	0.556	0.611	0
	SPTBN1	27	764	0	0	1	0	0	3.273
	THY	27	663	0	0	1	0	0	0
Sylvaticus clade	12SrRNA	27	593	27	0.01	13	0.961	5.81	4.64
	16SrRNA	27	347	23	0.02	14	0.974	6.843	3.361
	Cytochromeb	27	1,140	158	0.035	16	0.987	40.333	3.917
	MGF	27	671	10	0.002	4	0.399	1.601	6.246
	PRCK1	27	498	0	0	1	0	0	0
	SPTBN1	27	764	13	0.002	7	0.634	1.542	9.155
	THY	27	663	1	0.0003	2	0.209	0.209	4.785

Notes.

S

number of polymorphic sites

π

nucleotide diversity

h

number of haplotypes

Hd

haplotype diversity

k

average number of nucleotide differences

S/k

expansion coefficient

Statistically significant results were indicated by asterisks: *P < 0.05, **P < 0.01.