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. 2019 Apr 22;14(4):e0214440. doi: 10.1371/journal.pone.0214440

Table 2. Steady-state parameters of 5/B/6 MBL mutants.

Enzyme	Cefuroxime Hydrolysis
	No 10-mer^a			+ 500 μM 10-mer^a
	K_m (mM)	k_cat (s^-1)	k_cat/K_m (mM^-1·s^-1)	K_m (mM)	k_cat (s^-1)	k_cat/K_m (mM^-1·s^-1)
Wild type	1.2 ± 0.1	164 ± 8	133± 5	0.2 ± 0.1	36 ± 2	176 ± 30
K78Q	1.5 ± 0.1	117 ± 16	80 ± 7	1.2 ± 0.1	104± 6	89 ± 5
K104Q/K107Q	1.2 ± 0.1	154 ± 6	131 ± 7	1.2 ± 0.1	157 ± 11	126 ± 9
K78Q/K104Q/K107Q	1.3 ± 0.1	109 ± 10	82 ± 5	1.2 ± 0.1	109 ± 8	89 ± 9

^aKinetic values and associated errors represent the average of at least five replicates and their standard deviation.