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. 2019 Apr 22;14(4):e0215678. doi: 10.1371/journal.pone.0215678

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© 2019 Fathi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Morphological features of bone marrow derived-MSCs; (A) Spindle-shaped morphology of bone marrow cells that appear at day 1, (B) The number and size of colony appears to gradually increase on days 3–7, (C) More confluent bone marrow derived-MSCs at 21 days of culturing cells. (D) population doubling time (PDT) of bone marrow derived-MSCs. Two-lineage differentiation of bone marrow derived-MSCs; (E) Generation of lipid vacuoles after adipogenesis and staining by Sudan III; expression of PPAR-α and PPAR-γ as fat-specific genes, (F) Osteogenic differentiation and Alizarin red staining of mineralized cell aggregates; detection of the two bone specific genes (ALP and Runx2) by RT-PCR method. The undifferentiated cells were used as control in RT-PCR (scale bar = 40X).