Each cell surface marker was tested individually by separate cell population distributions and the isotopes controls were used as the negative control in this experiment; (A) a total population of cells for CD90 evaluation, (B) the bone marrow derived-MSCs were positive for CD90 (90.8%), (C) a total population of cells for CD44 evaluation, (D) the bone marrow derived-MSCs were positive for CD44 (84.6%), (E) a total population of cells for CD34 evaluation, (F) the bone marrow derived-MSCs were negative for CD34 (0.57%), (G) a total population of cells for CD56 evaluation, (H) the bone marrow derived-MSCs were negative for CD56 (0.23%). For CD34 and CD56, the isotype control was mouse IgG1 and for CD90 and CD44, the isotype control was mouse IgG2b. Also, isotype control is seen with red dots.