. Author manuscript; available in PMC: 2019 Dec 1.

Published in final edited form as: Toxicol Sci. 2018 Dec 1;166(2):318–331. doi: 10.1093/toxsci/kfy203

Table 2.

Xenopus laevis iodotyrosine deiodinase (IYD) primers and probes used to for RNA standard generation and qPCR.

Primer/Probe	Sequence (5’-3’)
RNA standard generation; outer forward	AAGGAAGAGTGGCAAGACCTAGAG
RNA standard generation; outer reverse	AAGATAAGGAATGGAGCTGTGTCC
RNA standard generation; middle forward	TAATACGACTCACTATAGGGAGGTTGTTGCACGTTCCATTTGC
RNA standard generation; middle reverse	CTAACCCACTTGTCTCCCATTCTT
qPCR forward	CATCAGAACAGCAGGGACATCTC
qPCR reverse	TGGGTCTTGCACAACAACAAA
qPCR probe	CAGTGGAGCCCATACTGAACCCTGGA