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. 2018 Dec 27;26(2):105–117. doi: 10.1093/dnares/dsy042

Figure 6.

Figure 6

Dynamic expression of lncRNAs during female germline development. (A) Dynamic expression of FGSC-specific highly expressed known lncRNAs during female germline development. Through the series cluster and tendency analysis, those FGSC-specific highly expressed known lncRNAs are shown. (B and C) Interaction network [including the miRNA-mRNA predicted relationship (Energy<−20, Score>170, P<0.001) and the lncRNA-miRNA interaction (Energy<−20, Score>155, P<0.001)] with WGCNA-derived hub genes at the FGSC stage. Partial enlargement of the interaction network with NCAPD2 (B) and CDKN1A(C) (WGCNA-derived hub genes at the FGSC stage) as representative examples. (D) XIST expression in FGSCs. I, single cells RT-PCR of XIST, TSIX, and MVH. Lanes 1-3, female tail fibroblasts (positive control); lanes 4-6, male tail fibroblasts (negative control); lanes 7-9, FGSCs; lanes 10-12, oocytes. II and III, FGSCs line showed the expected frequencies of the Xi-like enrichment for H3K27me3 (II). Female tail fibroblasts as a positive control (III). H3K27me3 (left) and DAPI (middle). Merge (right) of H3K27me3 and DAPI. Scale bars: 10 μm.