Fig. 2.

a Global gene expression profiles in the neural stem cells isolated from PRDX6 Tg mice. a Total RNA was obtained from the neural stem cells after 5 days of differentiation. Affymetrix GeneChip Mouse Gene 2.0 ST arrays containing 770, 317 mouse gene probes were used. Scatter plots show normalized intensities of each probe. b The expression of WDFY1 mRNA was determined by qPCR. c Western blotting. d Paraffin sections of brain from PRDX6 Tg or non-tg mice were stained with antibody against PRDX6. The data are expressed as the mean ± SD of three experiments. *P < 0.05 indicates significant difference from vector transfected neural stem cells. e PC12 cells were differentiated for 5 days upon stimulation with NGF (100 ng/ml) and neural stem cells were differentiated for 5 days; after that, we performed western blotting with WDFY1, TUBBIII, and NF antibodies