Fig. 4.

Effect of WDFY1 on the PRDX6-induced impaired differentiation of neural stem cells. a Neural stem cells transfected with WDFY1 siRNA, and then injected in SVZ region of ICR mice. After 2 weeks, differentiated astrocyte or neuronal cells were stained with GFAP or TUBBIII. b, c Neural stem cells isolated from non-tg or PRDX6 Tg mice were transfected with WDFY1 plasmid. Then, neural stem cells were differentiated into neuronal cells (b) and astrocytes (c) in vitro as described in the “Materials and Methods” section and then immunostained with beta III-tubulin or GFAP antibodies. β-actin was internal control. GFAP-positive cells and TUBBIII-positive neurite-bearing cells were quantified. The data are expressed as the mean ± SD of three experiments. *P < 0.05 indicates significant difference from vector-transfected neural stem cells. ^#P < 0.05 indicates significant difference from PRDX6 transfected neural stem cells